Melting dsDNA Donor Molecules Vastly Improves Precision Genome

November 6, 2020
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Melting dsDNA Donor Molecules Vastly Improves Precision Genome Enhancing in Caenorhabditiselegans

 

CRISPR genome modifying has revolutionized genetics in quite a few organisms. Contained in the nematode Caenorhabditiselegans one injection into every of the 2 gonad arms of an grownup hermaphrodite exposes tons of of meiotic germ cells to modifying mixtures, allowing the restoration of a whole lot of indels or small precision edits from every successfully injected animal. Sadly, significantly for extended insertions, modifying efficiencies can differ broadly, necessitating a whole lot of injections, and customarily requiring co-selection methods.

 

Correct proper right here we present that melting double stranded DNA (dsDNA) donor molecules earlier to injection will enhance the frequency of tangible homology-directed restore (HDR) by a whole lot of fold for longer edits. We describe troubleshooting methods that let persistently excessive modifying efficiencies ensuing, as an illustration, in as quite a bit as 100 unbiased GFP knock-ins from a single injected animal. These efficiencies make C. elegans by far the very best metazoan to genome edit, eradicating limitations to the use and adoption of this facile system as a mannequin for understanding animal biology.

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Description: A Rabbit polyclonal antibody against Mouse Vav 1 Oncogene (VAV1). This antibody is labeled with Cy3.

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Description: A Rabbit polyclonal antibody against Mouse Vav 1 Oncogene (VAV1). This antibody is labeled with HRP.

Vav 1 Oncogene (VAV1) Polyclonal Antibody (Human), FITC
4-PAC213Hu01-FITC
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Description: A Rabbit polyclonal antibody against Human Vav 1 Oncogene (VAV1). This antibody is labeled with FITC.

Vav 1 Oncogene (VAV1) Polyclonal Antibody (Mouse), FITC
4-PAC213Mu01-FITC
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Description: A Rabbit polyclonal antibody against Mouse Vav 1 Oncogene (VAV1). This antibody is labeled with FITC.

Vav 1 Oncogene (VAV1) Polyclonal Antibody (Human), Biotinylated
4-PAC213Hu01-Biotin
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Description: A Rabbit polyclonal antibody against Human Vav 1 Oncogene (VAV1). This antibody is labeled with Biotin.

Vav 1 Oncogene (VAV1) Polyclonal Antibody (Mouse), Biotinylated
4-PAC213Mu01-Biotin
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Description: A Rabbit polyclonal antibody against Mouse Vav 1 Oncogene (VAV1). This antibody is labeled with Biotin.

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Description: A Rabbit polyclonal antibody against Human Vav 1 Oncogene (VAV1). This antibody is labeled with APC-Cy7.

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Description: A Rabbit polyclonal antibody against Mouse Vav 1 Oncogene (VAV1). This antibody is labeled with APC-Cy7.

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EUR 355

Polyclonal Antibody to Vav 3 Oncogene (VAV3)
MBS2001848-1mL 1mL
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Water-Pipe Smoking Publicity Deregulates a Set of Genes Related to Human Head and Neck Most cancers Growth and Prognosis

 

  • Water-pipe smoking (WPS) is popping into in all probability probably the most well-liked form of tobacco use among the many many many youth, notably contained in the Coronary heart East, altering cigarettes quickly and turning into a vital threat of tobacco dependancy worldwide. Smoke from WPS accommodates comparable toxins as these current in cigarette smoke and is linked instantly with quite a few sorts of cancers together with lung and head and neck (HN) carcinomas.

 

  • Nonetheless, the underlying molecular pathways and/or function genes accountable for the carcinogenic course of are nonetheless unknown. On this research, human widespread oral epithelial (HNOE) cells, NanoStringPanCancer Pathways panel of 770 gene transcripts and quantitative real-time polymerase chain response (qRT-PCR) evaluation had been utilized to hunt out differentially expressed genes (DEG) modulated by WPS. In silico evaluation was carried out to analysis the have an effect on of those genes in HN most cancers affected specific individual’s biology and consequence. We discovered that WPS can induce the epithelial-mesenchymal transition (EMT: hallmark of most cancers development) of HNOE cells.

 

  • Additional considerably, our evaluation of NanoString revealed 23 genes deregulated beneath the have an effect on of WPS, accountable for the modulation of cell cycle, proliferation, migration/invasion, apoptosis, sign transduction, and inflammatory response. Additional evaluation was carried out utilizing qRT-PCR of HNOE WPS-exposed and unexposed cells supported the reliability of our NanoString data.

 

  • Furthermore, we exhibit these DEG to be upregulated in most cancers in distinction with widespread tissue. Utilizing the Kaplan-Meier evaluation, we seen a big affiliation between WPS-deregulated genes and relapse-free survival/full survival in HN most cancers victims. Our findings level out that WPS can modulate EMT together with a set of genes which could possibly be instantly concerned in human HN carcinogenesis, thereby affecting HN most cancers victims’ survival.

 

A pilot research on the genetic differ of Mycobacterium tuberculosis troublesome strains from tuberculosis victims contained in the Littoral area of Cameroon

Background: The re-emergence of tuberculosis (TB) worldwide, compounded by multi-drug resistance (MDR) of the causative brokers constitutes a big subject to the administration of the illness. Speedy analysis and correct stress identification are pivotal to the administration of the illness. This pilot research investigated the genetic differ of Mycobacterium tuberculosis troublesome (MTBC) strains from TB victims contained in the Littoral area of Cameroon together with their resistance to rifampicin (RIF).

 

Victims and methods: This was a cross sectional hospital-based research carried out between January and December 2017 and together with 158 isolates from sputum smear constructive people [105 (66.5%) males and 53 (33.5%) females]. Sputum samples had been examined utilizing Xpert MTB/RIF, adopted by customized on Lowenstein-Jensen medium. Isolates had been additional subjected to molecular characterization utilizing IS6110 typing, deletion evaluation and spoligotyping.

 

Outcomes: 13 (8.8%) of the 147 isolates with susceptibility outcomes accessible had been proof in direction of RIF. Drug resistance occurred in 5/50 (10%) feminine in contrast with 8/97 (8.2%) male (OR, 0.81; 0.25-2.62; p = 0.764), and there was no crucial distinction all via the age ranges (p = 0.448). Nonetheless, RIF resistance was related (OR, 0.18, 95%CI, 0.05-0.69; p = 0.023) with beforehand handled victims [(4/14 (28.6%)] in contrast with new ones [9/133 (6.8%)].

The 150 acknowledged lineages included amongst others 54 (36%) Cameroon, 18 (12%) UgandaI, 32 (21.3%) Haarlem, 17 (11.3%) Ghana, 9(6%) West African 1, 7(4.7%) Delhi/CAS, 4 (2.7%) LAM and three (2%) UgandaII. Of the 150 isolates, a really highly effective cluster was the Cameroon SIT 61, with 43(28.7%) isolates. Six (35.3%) of the 17 UgandaI sub-lineage had been RIF resistant (OR, 9.58; 95%CI, 2.74-33.55, p = 0.001).

 

Conclusion: The cosmopolitan Littoral area presents with a giant Mycobacterium tuberculosis (MTB) strains differ and the UgandaI sub-lineage attainable related to RIF resistance. Understanding the unfold of this clade via surveillance will improve TB administration contained in the area.

 

Marchantia polymorpha L. Flavonoids Shield Liver From CCl4-Induced Damage by Antioxidant and Gene-Regulatory Results

 

Marchantia polymorpha L. (MPL), a standard sort of liverwort, has been used as natural drugs to enhance liver perform in China for a few years. Though trendy research revealed that MPL incorporates numerous polyphenols, terpenoids, and bis[bibenzyls], its organic results on liver perform have by no means been systemically studied in any animal mannequin.
On this research, flavonoids had been extracted from MPL and the parts within the MPL flavonoids in addition to the antioxidant capability of MPL flavonoids had been analysed. A rat mannequin of liver harm was induced by intraperitoneal injection of 10% carbon tetrachloride (CCl4).
MPL flavonoids had been administered each day at a dose of 50, 100, and 200 mg/kg physique weight to the rats for two weeks previous to injection of CC14. Therapy with MPL flavonoids, particularly at a dose of 200 mg/kg, attenuated CCl4-induced will increase in alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, γ-glutamyl transpeptidase, nitric oxide, malondialdehyde, tumour necrosis factor-α, interleukin-1β, and interleukin-6, in addition to reductions in superoxide dismutase and glutathione peroxidase.
Microarray analyses confirmed that co-treatment with MPL flavonoids and CCl4 up-regulated many antioxidant and anti-apoptotic genes, however down-regulated a number of pro-inflammatory genes, in comparison with therapy with CCl4 alone. PCR and western blot assays additional recognized that MPL flavonoids elevated GPX1, TMX1, TXN, and XIAP expression, however decreased IL-1 and IL1RAP expression and inhibited Jak/stat3 signalling. In conclusion, MPL flavonoids exerted hepatoprotective results by way of antioxidant and gene regulatory mechanisms. (Altern Ther Well being Med.

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MBS2004826-02mL 0.2mL
EUR 225

Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2004826-05mL 0.5mL
EUR 380

Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2004826-1mL 1mL
EUR 465

Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2004826-5mL 5mL
EUR 1285

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat)
4-PAC578Ra01
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1)

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse)
4-PAC578Mu01
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1)

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), PE
4-PAC578Ra01-PE
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with PE.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), APC
4-PAC578Ra01-APC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with APC.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), Cy3
4-PAC578Ra01-Cy3
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with Cy3.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), HRP
4-PAC578Ra01-HRP
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with HRP.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), PE
4-PAC578Mu01-PE
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with PE.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), FITC
4-PAC578Ra01-FITC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with FITC.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), APC
4-PAC578Mu01-APC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with APC.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), Cy3
4-PAC578Mu01-Cy3
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with Cy3.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), HRP
4-PAC578Mu01-HRP
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with HRP.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), FITC
4-PAC578Mu01-FITC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with FITC.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), Biotinylated
4-PAC578Ra01-Biotin
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with Biotin.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), Biotinylated
4-PAC578Mu01-Biotin
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with Biotin.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), APC-Cy7
4-PAC578Ra01-APC-Cy7
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with APC-Cy7.

PE-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066795-01mL 0.1mL
EUR 265

PE-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066795-02mL 0.2mL
EUR 355

PE-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066795-05mL 0.5mL
EUR 660

PE-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066795-1mL 1mL
EUR 810

PE-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066795-5mL 5mL
EUR 2330

PE-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066801-01mL 0.1mL
EUR 275

PE-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066801-02mL 0.2mL
EUR 365

PE-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066801-05mL 0.5mL
EUR 675

PE-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066801-1mL 1mL
EUR 835

PE-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066801-5mL 5mL
EUR 2395

PE-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066807-01mL 0.1mL
EUR 285

PE-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066807-02mL 0.2mL
EUR 380

PE-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066807-05mL 0.5mL
EUR 710

PE-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066807-1mL 1mL
EUR 875

PE-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066807-5mL 5mL
EUR 2525

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat)
4-PAC578Hu01
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1)

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), APC-Cy7
4-PAC578Mu01-APC-Cy7
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with APC-Cy7.

APC-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066796-01mL 0.1mL
EUR 265

APC-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066796-02mL 0.2mL
EUR 355

APC-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066796-05mL 0.5mL
EUR 660

APC-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066796-1mL 1mL
EUR 810

APC-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066796-5mL 5mL
EUR 2330

Cy3-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066797-01mL 0.1mL
EUR 265

Cy3-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066797-02mL 0.2mL
EUR 355

Cy3-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066797-05mL 0.5mL
EUR 660

Cy3-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066797-1mL 1mL
EUR 810

Cy3-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066797-5mL 5mL
EUR 2330

HRP-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066799-01mL 0.1mL
EUR 205

HRP-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066799-02mL 0.2mL
EUR 255

HRP-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066799-05mL 0.5mL
EUR 450

HRP-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066799-1mL 1mL
EUR 545

HRP-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066799-5mL 5mL
EUR 1530

APC-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066802-01mL 0.1mL
EUR 275

APC-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066802-02mL 0.2mL
EUR 365

APC-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066802-05mL 0.5mL
EUR 675

APC-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066802-1mL 1mL
EUR 835

APC-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066802-5mL 5mL
EUR 2395

Cy3-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066803-01mL 0.1mL
EUR 275

Cy3-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066803-02mL 0.2mL
EUR 365

Cy3-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066803-05mL 0.5mL
EUR 675

Cy3-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066803-1mL 1mL
EUR 835

Cy3-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066803-5mL 5mL
EUR 2395

HRP-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066805-01mL 0.1mL
EUR 205

HRP-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066805-02mL 0.2mL
EUR 260

HRP-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066805-05mL 0.5mL
EUR 460

HRP-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066805-1mL 1mL
EUR 560

HRP-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066805-5mL 5mL
EUR 1570

APC-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066808-01mL 0.1mL
EUR 285

APC-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066808-02mL 0.2mL
EUR 380

APC-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066808-05mL 0.5mL
EUR 710

APC-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066808-1mL 1mL
EUR 875

APC-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066808-5mL 5mL
EUR 2525

Cy3-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066809-01mL 0.1mL
EUR 285

Cy3-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066809-02mL 0.2mL
EUR 380

Cy3-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066809-05mL 0.5mL
EUR 710

Cy3-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066809-1mL 1mL
EUR 875

Cy3-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066809-5mL 5mL
EUR 2525

HRP-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066811-01mL 0.1mL
EUR 215

HRP-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066811-02mL 0.2mL
EUR 270

HRP-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066811-05mL 0.5mL
EUR 485

HRP-Linked Polyclonal Antibody to Pim-1 Oncogene (PIM1)
MBS2066811-1mL 1mL
EUR 590
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