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The Genetic Diversification of a Single Bluetongue Virus Pressure Utilizing

November 6, 2020
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xanagen

The Genetic Diversification of a Single Bluetongue Virus Pressure Utilizing an In Vitro Mannequin of Alternating-Host Transmission

Bluetongue virus (BTV) is an arbovirus that has been related to dramatic epizootics in each wild and residential ruminants in latest a really very long time. As a segmented, double-stranded RNA virus, BTV can evolve by plenty of mechanisms attributable to its genomic constructing. Nonetheless, the have an effect on of BTV’s alternating-host transmission cycle on the virus’s genetic diversification stays poorly understood. Full genome sequencing approaches present a platform for investigating the have an effect on of host-alternation all by way of all ten segments of BTV’s genome.

 

To know the function of alternating hosts in BTV’s genetic diversification, a subject isolate was passaged beneath three totally completely totally different situations: (i) serial passages in Culicoidessonorensis cells, (ii) serial passages in bovine pulmonary artery endothelial cells, or (iii) alternating passages between insect and bovine cells.

 

Aliquots of virus had been sequenced, and single nucleotide variants had been acknowledged. Measures of viral inhabitants genetics had been used to quantify the genetic diversification that occurred. Two consensus variants in segments 5 and 10 occurred in virus from all three situations.

 

Whereas variants arose all by way of all passages, measures of genetic range remained largely comparable all by way of cell customized situations. Regardless of passage in a relaxed in vitro system, we discovered that this BTV isolate exhibited genetic stability all by way of passages and situations. Our findings underscore the expensive function that full genome sequencing might play in enhancing understanding of viral evolution and spotlight the genetic stability of BTV.

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MBS6122231-01mL MyBiosource 0.1(mL
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EUR 4050

EpCAM (CD326, Epithelial Cell Adhesion Molecule, Epithelial Specific Antigen) (PE)

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EpCAM (CD326, Epithelial Cell Adhesion Molecule, Epithelial Specific Antigen) (PE)

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Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1) (BSA/Azide Free) (Biotin)

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Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1) (BSA/Azide Free) (HRP)

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EUR 393.75

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Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1) (BSA/Azide Free) (MaxLight 650)

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MBS6267567-5x01mL MyBiosource 5x0.1mL
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Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1) (BSA/Azide Free) (MaxLight 550)

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Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1) (BSA/Azide Free) (MaxLight 550)

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Rat epithelial specific antigen(ESA) ELISA Kit

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Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human epithelial specific antigen,ESA ELISA Kit

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Human epithelial specific antigen (ESA) ELISA Kit

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Human epithelial specific antigen (ESA) ELISA Kit

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Human epithelial specific antigen (ESA) ELISA Kit

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Mouse epithelial specific antigen (ESA) ELISA Kit

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Mouse epithelial specific antigen (ESA) ELISA Kit

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Mouse epithelial specific antigen (ESA) ELISA Kit

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Human epithelial specific antigen, ESA ELISA Kit

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Human epithelial specific antigen, ESA ELISA Kit

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EUR 3420

Human epithelial specific antigen, ESA ELISA Kit

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Human epithelial specific antigen,ESA ELISA Kit

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Human epithelial specific antigen,ESA ELISA Kit

YLA2715HU-48T Shanghai YL Biotech 48T Ask for price

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Bovine epithelial specific antigen(ESA) ELISA Kit

EIA05626Bo Enlibio each
EUR 520

Canine epithelial specific antigen(ESA) ELISA Kit

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Rabbit epithelial specific antigen(ESA) ELISA Kit

EIA05626Rb Enlibio each
EUR 520

Bovine epithelial specific Antigen(ESA) ELISA Kit

MBS2609097-10x96StripWells MyBiosource 10x96-Strip-Wells
EUR 3545

Bovine epithelial specific Antigen(ESA) ELISA Kit

MBS2609097-48StripWells MyBiosource 48-Strip-Wells
EUR 310

Bovine epithelial specific Antigen(ESA) ELISA Kit

MBS2609097-5x96StripWells MyBiosource 5x96-Strip-Wells
EUR 1940

Bovine epithelial specific Antigen(ESA) ELISA Kit

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EUR 475

Rabbit epithelial specific antigen (ESA) ELISA Kit

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Rabbit epithelial specific antigen (ESA) ELISA Kit

MBS2601705-48StripWells MyBiosource 48-Strip-Wells
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Rabbit epithelial specific antigen (ESA) ELISA Kit

MBS2601705-5x96StripWells MyBiosource 5x96-Strip-Wells
EUR 1940

Rabbit epithelial specific antigen (ESA) ELISA Kit

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Canine epithelial specific antigen (ESA) ELISA Kit

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Canine epithelial specific antigen (ESA) ELISA Kit

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Canine epithelial specific antigen (ESA) ELISA Kit

MBS2604675-5x96StripWells MyBiosource 5x96-Strip-Wells
EUR 1940

Canine epithelial specific antigen (ESA) ELISA Kit

MBS2604675-96StripWells MyBiosource 96-Strip-Wells
EUR 475

Chicken epithelial specific antigen(ESA) ELISA Kit

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Porcine epithelial specific antigen(ESA) ELISA Kit

EIA05626p Enlibio each
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Porcine epithelial specific antigen (ESA) ELISA Kit

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Porcine epithelial specific antigen (ESA) ELISA Kit

MBS264398-48StripWells MyBiosource 48-Strip-Wells
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Porcine epithelial specific antigen (ESA) ELISA Kit

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EUR 1940

Porcine epithelial specific antigen (ESA) ELISA Kit

MBS264398-96StripWells MyBiosource 96-Strip-Wells
EUR 475

Chicken epithelial specific antigen (ESA) ELISA Kit

MBS265340-10x96StripWells MyBiosource 10x96-Strip-Wells
EUR 3545

Chicken epithelial specific antigen (ESA) ELISA Kit

MBS265340-48StripWells MyBiosource 48-Strip-Wells
EUR 310

Chicken epithelial specific antigen (ESA) ELISA Kit

MBS265340-5x96StripWells MyBiosource 5x96-Strip-Wells
EUR 1940

Chicken epithelial specific antigen (ESA) ELISA Kit

MBS265340-96StripWells MyBiosource 96-Strip-Wells
EUR 475

Epithelial Specific Antigen (ESA) Antibody, Concentrate

MAB431C Innovex 0.2ml
EUR 415

Human epithelial specific antigen-ESA-ELISA Kit

QY-E01682 Qayee Biotechnology 96T
EUR 400

Anti-Ep-CAM/Epithelial Specific Antigen antibody

STJ16100363 St John's Laboratory 1 mL
EUR 1041.6

Guinea pig epithelial specific antigen(ESA) ELISA Kit

EIA05626Gu Enlibio each
EUR 520

Guinea pig epithelial specific antigen (ESA) ELISA Kit

MBS260246-10x96StripWells MyBiosource 10x96-Strip-Wells
EUR 3545

Guinea pig epithelial specific antigen (ESA) ELISA Kit

MBS260246-48StripWells MyBiosource 48-Strip-Wells
EUR 310

Guinea pig epithelial specific antigen (ESA) ELISA Kit

MBS260246-5x96StripWells MyBiosource 5x96-Strip-Wells
EUR 1940

Guinea pig epithelial specific antigen (ESA) ELISA Kit

MBS260246-96StripWells MyBiosource 96-Strip-Wells
EUR 475

Epithelial Specific Antigen, (ESA) Antibody, Ready-To-Use

MAB431P Innovex 7ml
EUR 405

SensiStain Anti-Ep-CAM/Epithelial Specific Antigen Antibody

MBS4160459-01mg MyBiosource 0.1mg
EUR 320
×

 

 

Novel Gene Rearrangement and the Full Mitochondrial Genome of Cynoglossusmonopus: Insights into the Envolution of the Household Cynoglossidae (Pleuronectiformes)

 

Cynoglossusmonopus, a small benthic fish, belongs to the Cynoglossidae, Pleuronectiformes. It was not normally studied attributable to its low abundance and cryptical life-style. With the intention to know the mitochondrial genome and the phylogeny in Cynoglossidae, all of the mitogenome of C. monopus has been sequenced and analyzed for the primary time. The entire dimension is 16,425 bp, usually containing 37 genes with novel gene rearrangements. The tRNA-Gln gene is inverted from the sunshine to the heavy strand and translocated from the downstream of tRNA-Ile gene to its upstream. The administration area (CR) translocated downstream to the three’-end of ND1 gene adjoining to inverted to tRNA-Gln and left a 24 bp hint fragment inside the real place.

 

 

The phylogenetic timber had been reconstructed by Bayesian inference (BI) and most chance (ML) strategies primarily based completely on the mitogenomic information of 32 tonguefish species and two outgroups. The outcomes help the concept that Cynoglossidae is a monophyletic group and degree out that C. monopus has the closest phylogenetic relationship with C. puncticeps.

 

By combining fossil information and mitogenome information, the time-calibrated evolutionary tree of households Cynoglossidae and Soleidae was firstly offered, and it was indicated that Cynoglossidae and Soleidae had been differentiated from one another all by way of Paleogene, and the evolutionary technique of household Cynoglossidae coated the Quaternary, Neogene and Paleogene durations.

 

Genome-Broad Identification and Characterization of the SHI-Associated Sequence Gene Household in Rice

 

Rice (Oryzasativa) yield is correlated to varied components. Transcription regulators are essential components, akin to the regularly SHORT INTERNODES-related sequences (SRSs), which encode proteins with single zinc finger motifs. Nonetheless, information relating to the evolutionary and wise traits of the SRS gene household members in rice is inadequate.

 

Subsequently, we carried out a genome-wide screening and characterization of the OsSRS gene household in Oryzasativa japonica rice. We furthermore examined the SRS proteins from 11 rice sub-species, consisting of three cultivars, 6 wild varieties, and some completely totally different genome varieties. SRS members from maize, sorghum, Brachypodiumdistachyon, and Arabidopsis had been furthermore investigated.

 

All these SRS proteins exhibited species-specific traits, together with monocot- and dicot-specific traits, as assessed by phylogenetic evaluation, which was additional validated by gene constructing and motif analyses. Genome comparisons revealed that segmental duplications may have carried out essential roles contained in the recombination of the OsSRS gene household and their expression ranges. The household was primarily subjected to purifying selective stress.

 

Along with, the expression information demonstrated the distinct responses of OsSRS genes to varied abiotic stresses and hormonal cures, indicating their wise divergence. Our examine gives a beautiful reference for elucidating the choices of SRS genes in rice.

 

 

Src Inhibitor 1

MBS3607373-50mg MyBiosource 50mg
EUR 480

Src Inhibitor 1

MBS3607373-5mg MyBiosource 5mg
EUR 220

Src Inhibitor 1

T3593-10mg TargetMol Chemicals 10mg Ask for price
Description: Src Inhibitor 1

Src Inhibitor 1

T3593-1g TargetMol Chemicals 1g Ask for price
Description: Src Inhibitor 1

Src Inhibitor 1

T3593-1mg TargetMol Chemicals 1mg Ask for price
Description: Src Inhibitor 1

Src Inhibitor 1

T3593-50mg TargetMol Chemicals 50mg Ask for price
Description: Src Inhibitor 1

Src Inhibitor 1

T3593-5mg TargetMol Chemicals 5mg Ask for price
Description: Src Inhibitor 1

Src Inhibitor 3

HY-130254 MedChemExpress 1 mg
EUR 2380.99
Description: Src Inhibitor 3 is a potent, orally active c-terminal Src kinase (CSK) with IC50 values below 3 nM and 4 nM in CSK HTRF and Caliper assay, respectively. Src Inhibitor 3 shows the ability to increase T cell proliferation induced by T cell receptor signaling[1].

Src Inhibitor 3

MBS5767648-10mg MyBiosource 10mg
EUR 610

Src Inhibitor 3

MBS5767648-1mg MyBiosource 1mg
EUR 240

Src Inhibitor 3

MBS5767648-5mg MyBiosource 5mg
EUR 390

Src Inhibitor 3

T13000-10mg TargetMol Chemicals 10mg Ask for price
Description: Src Inhibitor 3

Src Inhibitor 3

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Description: Src Inhibitor 3

Src Inhibitor 3

T13000-1mg TargetMol Chemicals 1mg Ask for price
Description: Src Inhibitor 3

Src Inhibitor 3

T13000-50mg TargetMol Chemicals 50mg Ask for price
Description: Src Inhibitor 3

Src Inhibitor 3

T13000-5mg TargetMol Chemicals 5mg Ask for price
Description: Src Inhibitor 3

Jak/Src Inhibitor 1 hydrochloride

GW5327 Glentham Life Sciences 1mg
EUR 241.28

Jak/Src Inhibitor 1 hydrochloride

GW5327-1 Glentham Life Sciences 1
EUR 263.4

SRC kinase signaling inhibitor 1 (SRCIN1) Antibody

abx331175-100ul Abbexa 100 ul
EUR 510

SRC kinase signaling inhibitor 1 (SRCIN1) Antibody

abx327632-100g Abbexa 100 µg
EUR 250

SRC kinase signaling inhibitor 1 (SRCIN1) Antibody

20-abx327632 Abbexa
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  • 50 ug
  • 100 ug

SRC kinase signaling inhibitor 1 (SRCIN1) Antibody

abx327632-50g Abbexa 50 µg
EUR 187.5

Rat SRC kinase signaling inhibitor 1 (SRCIN1) ELISA Kit

abx392007-500l Abbexa 500 µl
EUR 687.5

Rat SRC kinase signaling inhibitor 1 (SRCIN1) ELISA Kit

abx392007-96tests Abbexa 96 tests
EUR 1093.2

Mouse SRC kinase signaling inhibitor 1 (SRCIN1) ELISA Kit

abx390644-500g Abbexa 500 µg
EUR 687.5

Mouse SRC kinase signaling inhibitor 1 (SRCIN1) ELISA Kit

abx390644-96tests Abbexa 96 tests
EUR 1093.2

Human SRC kinase signaling inhibitor 1 (SRCIN1) ELISA Kit

abx385443-96tests Abbexa 96 tests
EUR 687.5

Human SRC kinase signaling inhibitor 1, SRCIN1 ELISA KIT

ELI-13954h Lifescience Market 96 Tests
EUR 988.8

Mouse SRC kinase signaling inhibitor 1, Srcin1 ELISA KIT

ELI-18126m Lifescience Market 96 Tests
EUR 1038

SRCIN1 (untagged)-Human SRC kinase signaling inhibitor 1 (SRCIN1)

SC305252 Origene Technologies GmbH 10 µg Ask for price

Recombinant Rat SRC kinase signaling inhibitor 1 (Srcin1) , partial

MBS1354190-INQUIRE MyBiosource INQUIRE Ask for price

Recombinant Mouse SRC kinase signaling inhibitor 1 (Srcin1) , partial

MBS1395297-INQUIRE MyBiosource INQUIRE Ask for price

Recombinant Human SRC kinase signaling inhibitor 1 (SRCIN1) , partial

MBS1480399-INQUIRE MyBiosource INQUIRE Ask for price

SRCIN1 (GFP-tagged) - Human SRC kinase signaling inhibitor 1 (SRCIN1)

RG216673 Origene Technologies GmbH 10 µg Ask for price

Srcin1 ELISA Kit| Mouse SRC kinase signaling inhibitor 1 ELISA

EF016287 Lifescience Market 96 Tests
EUR 826.8

Srcin1 (untagged) - Mouse SRC kinase signaling inhibitor 1 (Srcin1), (10ug)

MC223972 Origene Technologies GmbH 10 µg Ask for price

Src, EGF, Non-competitive Inhibitor, Peptide A

MBS639302-1mg MyBiosource 1mg
EUR 610

Src, EGF, Non-competitive Inhibitor, Peptide A

MBS639302-5x1mg MyBiosource 5x1mg
EUR 2600

Srcin1 ELISA Kit| Rat SRC kinase signaling inhibitor 1 ELISA Ki

EF019367 Lifescience Market 96 Tests
EUR 826.8

Srcin1 (GFP-tagged) - Mouse SRC kinase signaling inhibitor 1 (Srcin1), (10ug)

MG222798 Origene Technologies GmbH 10 µg Ask for price

Srcin1 (Myc-DDK-tagged) - Mouse SRC kinase signaling inhibitor 1 (Srcin1)

MR222798 Origene Technologies GmbH 10 µg Ask for price

SRCIN1 (Myc-DDK-tagged)-Human SRC kinase signaling inhibitor 1 (SRCIN1)

RC216673 Origene Technologies GmbH 10 µg Ask for price

Lenti ORF clone of Human SRC kinase signaling inhibitor 1 (SRCIN1), mGFP tagged

RC216673L4 Origene Technologies GmbH 10 µg Ask for price

Lenti ORF clone of Srcin1 (mGFP-tagged) - Mouse SRC kinase signaling inhibitor 1 (Srcin1)

MR222798L4 Origene Technologies GmbH 10 µg Ask for price

Lenti ORF clone of Human SRC kinase signaling inhibitor 1 (SRCIN1), Myc-DDK-tagged

RC216673L3 Origene Technologies GmbH 10 µg Ask for price

Lenti ORF clone of Srcin1 (Myc-DDK-tagged) - Mouse SRC kinase signaling inhibitor 1 (Srcin1)

MR222798L3 Origene Technologies GmbH 10 µg Ask for price

Lenti ORF particles, Srcin1 (GFP-tagged) - Mouse SRC kinase signaling inhibitor 1 (Srcin1), 200ul, >10^7 TU/mL

MR222798L4V Origene Technologies GmbH 200 µl Ask for price

Lenti ORF particles, SRCIN1 (mGFP-tagged) - Human SRC kinase signaling inhibitor 1 (SRCIN1), 200ul, >10^7 TU/mL

RC216673L4V Origene Technologies GmbH 200 µl Ask for price

Lenti ORF particles, SRCIN1 (Myc-DDK tagged) - Human SRC kinase signaling inhibitor 1 (SRCIN1), 200ul, >10^7 TU/mL

RC216673L3V Origene Technologies GmbH 200 µl Ask for price

Lenti ORF particles, Srcin1 (Myc-DDK-tagged) - Mouse SRC kinase signaling inhibitor 1 (Srcin1), 200ul, >10^7 TU/mL

MR222798L3V Origene Technologies GmbH 200 µl Ask for price

Antizyme Inhibitor 1 (Antizyme Inhibitor 1) Antibody

20-abx111016 Abbexa
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  • 50 ul
  • 150 ul

Antizyme Inhibitor 1 (Antizyme inhibitor 1) Antibody

abx230450-100ug Abbexa 100 ug
EUR 577.2

NDM-1 inhibitor-1

561825 MedKoo Biosciences 5.0mg
EUR 340

NDH-1 inhibitor-1

HY-148441 MedChemExpress 10 mg
EUR 324.68
Description: NDH-1 inhibitor-1 (compound 27) is a potent NDH-1 inhibitor with pI50 values of E. coli, respectively[1].

NDM-1 inhibitor-1

MBS5777843-5mg MyBiosource 5(mg
EUR 915

NDM-1 inhibitor-1

MBS5777843-5x5mg MyBiosource 5x5(mg
EUR 3970

HIF-1 inhibitor-1

T36290-10mg TargetMol Chemicals 10mg Ask for price
Description: HIF-1 inhibitor-1

HIF-1 inhibitor-1

T36290-1g TargetMol Chemicals 1g Ask for price
Description: HIF-1 inhibitor-1

HIF-1 inhibitor-1

T36290-1mg TargetMol Chemicals 1mg Ask for price
Description: HIF-1 inhibitor-1

HIF-1 inhibitor-1

T36290-50mg TargetMol Chemicals 50mg Ask for price
Description: HIF-1 inhibitor-1

HIF-1 inhibitor-1

T36290-5mg TargetMol Chemicals 5mg Ask for price
Description: HIF-1 inhibitor-1

NDM-1 inhibitor-1

T28148-10mg TargetMol Chemicals 10mg Ask for price
Description: NDM-1 inhibitor-1

NDM-1 inhibitor-1

T28148-1g TargetMol Chemicals 1g Ask for price
Description: NDM-1 inhibitor-1

NDM-1 inhibitor-1

T28148-1mg TargetMol Chemicals 1mg Ask for price
Description: NDM-1 inhibitor-1

NDM-1 inhibitor-1

T28148-50mg TargetMol Chemicals 50mg Ask for price
Description: NDM-1 inhibitor-1

NDM-1 inhibitor-1

T28148-5mg TargetMol Chemicals 5mg Ask for price
Description: NDM-1 inhibitor-1

ATM Inhibitor-1

HY-112614 MedChemExpress Get quote Ask for price
Description: ATM Inhibitor-1 is a highly potent, selective and orally active ATM inhibitor, with an IC50 of 0.7 nM, shows weak activity against mTOR (IC50, 21 μM), DNAPK (IC50, 2.8 μM), PI3Kα (IC50, 3.8 μM), PI3Kβ (IC50, 10.3 μM), PI3Kγ (IC50, 3 μM) and PI3Kδ (IC50, 0.73 μM). ATM Inhibitor-1 exhibits anti-tumor activity[1].

Cot inhibitor-1

HY-32015 MedChemExpress 10mg
EUR 1753.27
Description: Cot inhibitor-1 (compound 28) is a selective tumor progression loci-2 (Tpl2) kinase inhibitor with an IC50 of 28 nM. Cot inhibitor-1 shows an inhibition of TNF-alpha production in human whole blood with an IC50 of 5.7 nM[1].

EMT inhibitor-1

HY-101275 MedChemExpress 5mg
EUR 1396.13
Description: EMT inhibitor-1 is an inhibitor of of Hippo, TGF-β, and Wnt signaling pathways with antitumor activities.

sEH inhibitor-1

HY-120494 MedChemExpress 1 mg
EUR 1136.38
Description: sEH inhibitor-1 (compound TCPU ) is a potent and oral active inhibitor of sEH (soluble epoxide hydrolase) with IC50s of 0.4 and 5.3 nM in human and murine, respectively[1].

Cot inhibitor-1

MBS386730-10mg MyBiosource 10mg
EUR 545

Cot inhibitor-1

MBS386730-2mg MyBiosource 2mg
EUR 200

Cot inhibitor-1

MBS386730-50mg MyBiosource 50mg
EUR 1485

Cot inhibitor-1

MBS386730-5mg MyBiosource 5mg
EUR 305

Cot inhibitor-1

MBS386730-5x50mg MyBiosource 5x50mg
EUR 6665

EMT inhibitor-1

MBS3844872-100mg MyBiosource 100mg
EUR 4815

EMT inhibitor-1

MBS3844872-10mg MyBiosource 10mg
EUR 865

EMT inhibitor-1

MBS3844872-50mg MyBiosource 50mg
EUR 2450

EMT inhibitor-1

MBS3844872-5mg MyBiosource 5mg
EUR 525

EMT inhibitor-1

MBS3844872-5x100mg MyBiosource 5x100mg
EUR 21665

ATM Inhibitor-1

MBS3844383-10mg MyBiosource 10mg
EUR 1905

ATM Inhibitor-1

MBS3844383-1mg MyBiosource 1mg
EUR 430

ATM Inhibitor-1

MBS3844383-5mg MyBiosource 5mg
EUR 1165

ATM Inhibitor-1

MBS3844383-5x10mg MyBiosource 5x10mg
EUR 8565

Cot inhibitor-1

MBS5752943-100mg MyBiosource 100mg
EUR 2520

Cot inhibitor-1

MBS5752943-50mg MyBiosource 50mg
EUR 1570

Cot inhibitor-1

MBS5752943-5x100mg MyBiosource 5x100mg
EUR 11200

EMT inhibitor-1

MBS5753091-25mg MyBiosource 25mg
EUR 1740

EMT inhibitor-1

MBS5753091-5x25mg MyBiosource 5x25mg
EUR 7675

ATM Inhibitor-1

MBS5766091-5mg MyBiosource 5mg
EUR 915
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